(wash) stringency

Dutch translation: stringentie (tijdens de wasprocedure)

GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW)
English term or phrase:(wash) stringency
Dutch translation:stringentie (tijdens de wasprocedure)
Entered by: Michiel Leeuwenburgh

13:39 Nov 2, 2011
English to Dutch translations [PRO]
Science - Biology (-tech,-chem,micro-) / moleculaire biologie, DNA-hydbridisatie
English term or phrase: (wash) stringency
In een octrooitekst over DNA-constructen:
For example, hybridization of such sequences can be carried out under conditions of reduced stringency, medium stringency or even stringent conditions (e.g., conditions represented by a wash stringency of 35-40% formamide with 5x Denhardt’s solution, 0.5% SDS and 1x SSPE at 37°C; conditions represented by a wash stringency of 40-45% formamide with 5x Denhardt’s solution, 0.5% SDS, and 1x SSPE at 42°C; and conditions represented by a wash stringency of 50% formamide with 5x Denhardt’s solution, 0.5% SDS and 1x SSPE at 42°C, respectively).
Ik zie hier en daar dat *stringency* vertaald wordt met *striktheid*, maar ik ben er niet zeker van of dit in het vakgebied gangbaar is. Nog minder zeker ben ik van een vertaling van *wash stringency* (*wasstriktheid* bestaat in ieder geval niet...).
Alle suggesties zijn welkom!
Michiel Leeuwenburgh
Netherlands
Local time: 08:42
stringentie (tijdens de wasprocedure)
Explanation:
Niet echt veel referenties, maar laagstringente, lage stringentie en hoogstringente vind ik een paar keer (misschien het meeste in België)
Selected response from:

Koen Speetjens (X)
Local time: 08:42
Grading comment
Hartelijk bedankt, Koen!
4 KudoZ points were awarded for this answer



Summary of answers provided
2 +3stringentie (tijdens de wasprocedure)
Koen Speetjens (X)
3precizie
Machteld/Johan Schrameijer/Westenburg
Summary of reference entries provided
definition
Barend van Zadelhoff

  

Answers


16 mins   confidence: Answerer confidence 2/5Answerer confidence 2/5 peer agreement (net): +3
stringentie (tijdens de wasprocedure)


Explanation:
Niet echt veel referenties, maar laagstringente, lage stringentie en hoogstringente vind ik een paar keer (misschien het meeste in België)


    Reference: http://www.nvkc.nl/tijdschrift/content/1998/nr%206/p249/1998...
    Reference: http://epatras.economie.fgov.be/DisplayPDF/ExtraData/NL/2187...
Koen Speetjens (X)
Local time: 08:42
Native speaker of: Native in DutchDutch
PRO pts in category: 4
Grading comment
Hartelijk bedankt, Koen!

Peer comments on this answer (and responses from the answerer)
agree  Ron Hartong PhD (X): Ik zie dat je me voor bent geweest - ik laat mijn antwoord een tijdje staan en trek het dan terug ;)
3 mins

agree  InekeTromp (X): en zie ook deze link http://www.uyttebroek.com/jan/KD_Industria/1334/1334_MolecCe...
10 mins

agree  Marjolein Snippe
17 mins
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16 mins   confidence: Answerer confidence 3/5Answerer confidence 3/5
precizie


Explanation:
Of graad van preciezie kan hier mischien goed passen.
(spelling?)

Machteld/Johan Schrameijer/Westenburg
Local time: 08:42
Native speaker of: Native in DutchDutch, Native in EnglishEnglish
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Reference comments


32 mins
Reference: definition

Reference information:
Stringency - a term used in hybridization experiments to denote the degree of homology between the probe and the filter bound nucleic acid; the higher the stringency, the higher percent homology between the probe and filter bound nucleic acid

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The next step in a hybridization experiment is to wash the filter. This is normally done in two steps. First a non-stringent wash is performed to remove the non-specifically bound DNA and the second wash is performed at a higher stringency that only permits highly homologous sequences to remain bound to the filter. Controlling the stringency is an important step in these experiments

http://www.ndsu.edu/pubweb/~mcclean/plsc731/dna/dna6.htm

Barend van Zadelhoff
Netherlands
Native speaker of: Native in DutchDutch
PRO pts in category: 32
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