20:10 Aug 21, 2019 |
English to German translations [PRO] Medical - Biology (-tech,-chem,micro-) | |||||||
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| Selected response from: D. I. Verrelli Australia Local time: 05:07 | ||||||
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Summary of answers provided | ||||
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3 | GPO mit Triglycerid-Leerwert |
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3 | "Triglyceride, nach GPO-Verfahren mit Leerwertkorrektur" |
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Summary of reference entries provided | |||
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enzyme glycerol phosphate oxidase (GPO) method and sample to cuvet labeled Blank |
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Discussion entries: 2 | |
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triglycerides gpo blanked GPO mit Triglycerid-Leerwert Explanation: Glycerin-3-phosphat-Dehydrogenase (GPD) heißen Enzyme, die die Hydrierung von Dihydroxyacetonphosphat (DHAP) zu Glycerin-3-phosphat und die umgekehrte Umsetzung katalysieren. "Bei den Assay-Parametern für Triglyceride-Leerwert (TG-B) handelt es sich um einen alternativen Parametersatz zur Verwendung mit dem Triglyceride-GPO-Reagenz (TG)." Siehe auch http://www.biolabo.fr/biolabo/pdfs/noticesE/biochimieE/AT-80... -------------------------------------------------- Note added at 15 Stunden (2019-08-22 11:19:27 GMT) -------------------------------------------------- oder auch: Blindprobe -------------------------------------------------- Note added at 15 Stunden (2019-08-22 11:19:48 GMT) -------------------------------------------------- bzw. Blindwert |
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triglycerides GPO blanked "Triglyceride, nach GPO-Verfahren mit Leerwertkorrektur" Explanation: See Discussion section. https://www.beckmancoulter.com/download/file/phxA18555AR-DE_... https://www.beckmancoulter.com/wsrportal/techdocs?docname=/c... -------------------------------------------------- Note added at 1 day 6 hrs (2019-08-23 02:56:30 GMT) -------------------------------------------------- If you want to follow the Beckman–Coulter nomenclature, then it would be "Triglyceride-GPO (Leerwert)". Perhaps that's a good option for laboratory staff or other experts working in that field. For anyone else I think it would be rather hard to interpret. By my reading of the Beckman–Coulter method statements, what this is actually intended to mean is the level/concentration of triglycerides, according to the following technique: (i) Through use of a "triglycerides GPO reagent" that will convert triglycerides to glycerol, determine the apparent content of triglycerides in a synthetic sample [the "blank"] that is known to contain no triglycerides, but instead contains glycerol at an appropriate concentration. (ii) Through use of a "triglycerides GPO reagent" that will convert triglycerides to glycerol, determine the apparent content of triglycerides in a real (biological) sample that is expected to contain both triglycerides and glycerol. (iii) Estimate the true content of triglycerides in a real (biological) sample by subtracting the result of analysis (i) from the result of analysis (ii). I'm not entirely sure, but it seems that the "triglycerides GPO reagent" should contain a few things: "Triglycerides in the sample are hydrolyzed to glycerol and free fatty acids by the action of lipase. A sequence of three coupled enzymatic steps using glycerol kinase (GK), glycerophosphate oxidase (GPO), and horseradish peroxidase (HPO) causes the oxidative coupling [...] to form a red [...] dye." -------------------------------------------------- Note added at 1 day 6 hrs (2019-08-23 03:00:31 GMT) -------------------------------------------------- ...So I don't think the reported value is the concentration of GPO present (I think GPO is a component of the "reagent"), nor do I think the blank/control value is being reported (I think the blank value has been applied to obtain the reported value). -------------------------------------------------- Note added at 1 day 6 hrs (2019-08-23 03:08:05 GMT) -------------------------------------------------- Amusingly, I came up with "Leerwertkorrektur" before discovering that it is a term often used by Beckman–Coulter! https://www.linguee.com/english-german/search?source=auto&qu... I wouldn't be surprised if there are some other more idiomatic options for this portion of the translation. Borrowing an idea from Barbara Schmidt, "Blindwertkorrektur" is used sometimes https://www.linguee.com/english-german/search?source=auto&qu... Still not very often, yet significantly more often than "Leerwertkorrektur". -------------------------------------------------- Note added at 1 day 6 hrs (2019-08-23 03:09:52 GMT) -------------------------------------------------- "[...] intended for quantitative determination of triglycerides concentration in human serum or plasma." "[...] dient [...] der quantitativen Bestimmung der Konzentration an Triglyceriden in Humanserum oder -plasma." -------------------------------------------------- Note added at 1 day 8 hrs (2019-08-23 04:56:43 GMT) -------------------------------------------------- I acknowledge Cornelia Litzmann's helpful post on the TranslatorsCafé forum. |
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Reference: enzyme glycerol phosphate oxidase (GPO) method and sample to cuvet labeled Blank Reference information: Triglycerides (GPO) Reagent Set - Pointe Scientific https://www.pointescientific.com/uploads/inserts/OT732-01-12... Test Summary. The triglycerides (GPO) method is based on the enzymatic determination of glycerol using the enzyme glycerol phosphate oxidase (GPO) after ... Free Glycerol Determination Kit (FG0100) - Technical Bulletin coralreefdiagnostics.com/s/Sigma-glycerol.pdf quantitative enzymatic determination of glycerol in serum or plasma at ... endogenous glycerol using the same coupled enzyme reactions ... glycerol phosphate oxidase (GPO) to .... and sample to cuvets labeled Blank, Standard, and Sample ... -------------------------------------------------- Note added at 2 hrs (2019-08-21 22:44:07 GMT) -------------------------------------------------- Triglycerides GPO-POD. Liquid - Atlas Medical atlas-medical.com/upload/.../Triglycerides%20Package%20Insert.pdf Sample triglycerides incubated with lipoproteinlipase (LPL), liberate glycerol and free fatty acids. Glycerol is converted to glycerol-3-phosphate (G3P) and adenosine- ... Glycerol-3-oxidase (GPO) ... expiration date on the label when stored tightly ... to zero with distilled water. 3. Pipette into a cuvette: Blank. Standard. Sample. |
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