restriction digestion

Lithuanian translation: virškinimas apribojant Dde I

GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW)
English term or phrase:Dde I restriction digestion
Lithuanian translation:virškinimas apribojant Dde I
Entered by: Kristina Radziulyte

07:46 Jul 31, 2012
English to Lithuanian translations [PRO]
Science - Biology (-tech,-chem,micro-)
English term or phrase: restriction digestion
We used Dde I enzyme to determine the heterogeneity of campylobacter strains isolated in broilers because Dde I restriction digestion provides the best discrimination for veterinary isolates
Irena R
Local time: 19:01
virškinimas apribojant Dde I
Explanation:
Abstract

Protein 4.1 is a major component of the junctional complex at the red cell skeleton. Genomic studies have recently evidenced that the encoding gene (EL1 locus) is present in a single copy per haploid genome. Several RFLPs have already been characterized within intron sequences. Here, we describe the first RFLP found within the coding sequence. This polymorphism (C or T at position 2723, in exon 21) does not affect the amino acid sequence (Thr-->Thr). It can be detected by either Dde I restriction digestion of an appropriate PCR product, or simply by SSCP These findings should facilitate analysis of families with 4.1 deficiencies causing hereditary elliptocytosis.

tHIS ABstract from http://www.ncbi.nlm.nih.gov/pubmed/9554757 shows that the words "Dde I restriction digestion of an appropriate PCR product" belong together in the phrase just mentioned.
Selected response from:

Gintautas Kaminskas
Australia
Local time: 04:01
Grading comment
Ačiū.
4 KudoZ points were awarded for this answer



Summary of answers provided
2virškinimas apribojant Dde I
Gintautas Kaminskas


  

Answers


21 mins   confidence: Answerer confidence 2/5Answerer confidence 2/5
Dde I restriction digestion
virškinimas apribojant Dde I


Explanation:
Abstract

Protein 4.1 is a major component of the junctional complex at the red cell skeleton. Genomic studies have recently evidenced that the encoding gene (EL1 locus) is present in a single copy per haploid genome. Several RFLPs have already been characterized within intron sequences. Here, we describe the first RFLP found within the coding sequence. This polymorphism (C or T at position 2723, in exon 21) does not affect the amino acid sequence (Thr-->Thr). It can be detected by either Dde I restriction digestion of an appropriate PCR product, or simply by SSCP These findings should facilitate analysis of families with 4.1 deficiencies causing hereditary elliptocytosis.

tHIS ABstract from http://www.ncbi.nlm.nih.gov/pubmed/9554757 shows that the words "Dde I restriction digestion of an appropriate PCR product" belong together in the phrase just mentioned.

Gintautas Kaminskas
Australia
Local time: 04:01
Native speaker of: Native in LithuanianLithuanian
PRO pts in category: 12
Grading comment
Ačiū.
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