Feb 3, 2010 14:01
14 yrs ago
2 viewers *
Spanish term

desalados

Spanish to English Medical Science (general)
Los oligonucleótidos (primers) para PCR múltiple están basados en secuencias de nucleótidos publicados y flanquean diferentes secuencias en el genoma del género Brucella. Pedir desalados y desprotegidos, a 100 nmol o 200nmol.
Proposed translations (English)
5 desalted
2 +4 desalinated
5 salt-free

Discussion

Dolores Arjo (X) Feb 3, 2010:
salt-free it means that salt was removed in the purification process for the oligo. I have never seen desalinated applied to primers
Rafael Geronimo Feb 3, 2010:
In my opinion, Desalinated and Salt-free are not the same. The first one implies that the salt content was removed it and Salt-free may imply that there never was salt content

Proposed translations

1 day 16 hrs
Selected

desalted

Liz is correct. 'Desalinated' appears to be a calque:

102,000 refs for [oligonucleotides+desalted]
22,000 for [oligonucleotides+desalinated] - and they appear to be translations or texts by non-native speakers

Protocol for Desalting Oligonucleotides Using Butanol Extraction http://www.synthegen.com/technical/protocols/butan... Added: Sun Oct 26 2003, Hits: 310, ...
www.protocol-online.org/.../Oligonucleotide/Oligo.../index.... -

High-throughput biopolymer desalting by solid-phase extraction ...
by M Gilar - 2001 - Cited by 22 - Related articles
In this paper we describe the development of a rapid and effective RP-SPE method for DNA oligonucleotide and peptide desalting. The selection of the type of ...
linkinghub.elsevier.com/retrieve/pii/S0021967301008330

At Sigma-Genosys, every oligo is desalted. Desalting removes residual by-products from the synthesis, cleavage, and deprotection procedures. ...
www.sigmaaldrich.com/img/assets/19000/purification1.pdf

Explanation of Oligonucleotide Synthesis
Figure 8. An example of a oligonucleotide. Image used with permission from Integrated DNA Technologies. Desalting. Desalting is done to purify the solution. ...
www.bio.davidson.edu/.../oligonucleotide_synthesis.htm

Desalting Oligonucleotides by Gel Filtration
When the 1.5 ml has entirely run through the column, the salt removal is complete. The desalted oligonucleotide is now ready to be dried or quantitated. ...
www.bioon.com.cn/protocol/showarticle.asp?newsid=1365 -

It has been specifically developed by Eurogentec to increase the purity level of the deprotected and desalted Oligonucleotides from 15 to 80 bases. ...
www.eurogentec.com/product/research-sepop-desalting.html -

Custom primers (Oligo synthesis) for Sequencing - Bionexus Oligo ...
Desalting is desirable to remove protecting groups and residual salts that can affect the biological activity of the oligonucleotide. ...
www.bionexus.net/oligo_synthesis.html

Desalting of peptide and protein solutions
The first example illustrates the fact that RPC can be an excellent desalting method for synthetic oligonucleotides. Figure 1 demonstrates the desalting of ...
www.amberchrom.com/desalting.htm
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4 KudoZ points awarded for this answer. Comment: "Many thanks to all for your help"
+4
10 mins

desalinated

desalinated
Peer comment(s):

agree liz askew : it is actually "desalted" from "desalar":http://www.google.co.uk/search?hl=en&q=desalted oligonucleot...|countryGB&aq=f&oq=
10 mins
agree Rafael Geronimo
1 hr
agree Richard McDorman
1 hr
agree sugrass : "Desalted".
7 hrs
Something went wrong...
56 mins

salt-free

salt-free and unmodified
(mira en la página de cualquier compañia de diseño de primers, como operon)
Peer comment(s):

neutral Richard McDorman : Wouldn't Spanish probably use "sin sal" for "salt-free"?
42 mins
no es una traduccion literal. Es la terminología científica utilizada para este tipo de primers
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Reference comments

3 hrs
Reference:

#
he role of phosphate groups in the VS ribozyme–substrate interaction
by YS Kovacheva - 2004 - Related articles
The chemical homogeneity of the purified and desalted oligonucleotides was examined by MALDI-TOF mass-spectrometry (Brüker Reflex III MALDI-TOF instrument; ...
ukpmc.ac.uk › Journal List › Nucleic Acids Res › v.32(21); 2004
Show more results from ukpmc.ac.uk
#
ABgene - Press Release details
From expression analysis to gene silencing, biomers.net have the expertise to generate high purity, desalted oligonucleotides to ensure reproducible results ...
www.abgene.com/PRDetails.asp?PRID=4 - Cached
# [PDF]
Synthesis of UNA and chimeric UNA/DNA, UNA/DNA/RNA ...
File Format: PDF/Adobe Acrobat
After deprotection the oligonucleotides were desalted by G25 and quantified. The crude oligonucleotides were then analysed by RP-HPLC on an Agilent Eclipse ...
www.linktech.co.uk/.../311_synthesis-of-una-and-chimeric-un...
#
Severn Biotech Ltd
Oligonucleotides undergo UV analysis to assess the quantity of the product and to ensure the salt content has been eliminated by the desalting process which ...
www.severnbiotech.com › Categories - Cached - Similar
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