Glossary entry (derived from question below)
English term or phrase:
boomerang DNA amplification
Portuguese translation:
amplificação de DNA tipo bumerangue
Added to glossary by
Clauwolf
Mar 5, 2019 03:10
5 yrs ago
English term
United States
Translator/Chemist/Engineer (BSc/MSc)
Native in: Portuguese (Variant: Brazilian) 
Works in: English to Portuguese, Portuguese to English, Italian to Portuguese
boomerang DNA amplification
English to Portuguese
Science
Biology (-tech,-chem,micro-)
Non-limiting examples of nucleic acid amplification techniques include polymerase chain reaction (PCR), reverse transcriptase polymerase chain reaction (RT-PCR), nested PCR, ligase chain reaction (see Abravaya, K. et al., Nucleic Acids Res.
(1995), 23:675-682), branched DNA signal amplification (see Urdea, M. S. et al., AIDS (1993), 7(suppl 2):S11- S14), amplifiable RNA reporters, Q-beta replication, transcription-based amplification, boomerang DNA amplification, strand displacement activation, cycling probe technology, isothermal nucleic acid sequence based amplification (NASBA) (see Kievits, T. et al., J Virological Methods (1991), 35:273-286), Invader Technology, next-generation sequencing technology or other sequence replication assays or signal amplification assays.
(1995), 23:675-682), branched DNA signal amplification (see Urdea, M. S. et al., AIDS (1993), 7(suppl 2):S11- S14), amplifiable RNA reporters, Q-beta replication, transcription-based amplification, boomerang DNA amplification, strand displacement activation, cycling probe technology, isothermal nucleic acid sequence based amplification (NASBA) (see Kievits, T. et al., J Virological Methods (1991), 35:273-286), Invader Technology, next-generation sequencing technology or other sequence replication assays or signal amplification assays.
Proposed translations
(Portuguese)
| 3 +2 | amplificação de DNA tipo bumerangue |
Clauwolf
|
| 4 | amplificação bumerangue de DNA |
Monica Mazzoni
|
Change log
Mar 5, 2019 12:18: Matheus Chaud changed "Term asked" from "boomerang DNA amplificatio" to "boomerang DNA amplification"
Apr 2, 2019 12:14: Clauwolf Created KOG entry
Proposed translations
+2
9 hrs
Selected
amplificação de DNA tipo bumerangue
:) boomerang DNA amplification (BDA)
A technique similar to PCR that also uses a heat-resistant polymerase and cycles of polymerization, denaturation and annealing, but which requires only one primer. The source DNA is digested with a restriction endonuclease. A universal adaptor is engineered with self-complementary sections, so that it loops back upon itself and has ends that permit ligation to both strands at each end of the restriction fragment. In the first amplification cycle a primer anneals to an internal site and the polymerase copies the primer-binding strand through the adaptor, back along the second strand and past the site that is complementary to the primer-binding site of the first strand. In subsequent cycles the primer will have two sites for initiation of polymerization. Hengen, P.N. (1995) Trends Biochem. Sci. 20, 372-373
A technique similar to PCR that also uses a heat-resistant polymerase and cycles of polymerization, denaturation and annealing, but which requires only one primer. The source DNA is digested with a restriction endonuclease. A universal adaptor is engineered with self-complementary sections, so that it loops back upon itself and has ends that permit ligation to both strands at each end of the restriction fragment. In the first amplification cycle a primer anneals to an internal site and the polymerase copies the primer-binding strand through the adaptor, back along the second strand and past the site that is complementary to the primer-binding site of the first strand. In subsequent cycles the primer will have two sites for initiation of polymerization. Hengen, P.N. (1995) Trends Biochem. Sci. 20, 372-373
4 KudoZ points awarded for this answer.
Comment: "Selected automatically based on peer agreement."
2 days 16 hrs
Monica Mazzoni
Brazil
Brazil
Native in Portuguese
Brazil
Native in: Portuguese (Variant: Brazilian) 
Works in: English to Portuguese, Portuguese to English
amplificação bumerangue de DNA
Acho que assim fica super claro que o bumerangue se refere ao método e não ao DNA.
Outra forma de referir-se ao método, mas que não cabe na frase colocada pela colega Isadora, seria método bumerangue de amplificação do DNA.
A frase de exemplo explica bem a razão para utilizar o termo boomerang.
Outra forma de referir-se ao método, mas que não cabe na frase colocada pela colega Isadora, seria método bumerangue de amplificação do DNA.
A frase de exemplo explica bem a razão para utilizar o termo boomerang.
Example sentence:
The procession of replication around the spacer 100 suggested the motion of a boomerang, hence the coined name of the present method.
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