Apr 11 11:42
1 mo ago
11 viewers *
English term
low brake
English to Polish
Science
Medical: Pharmaceuticals
badania krwi
A patient sample that was stored (12 – 72 hours), should be washed three times using 1x reagent D (3 x 2 mL at 300 g for 3 minutes, low brake) before starting the tests.
Cord and adult blood should always be washed three times using 1x reagent D (3 x 2 mL at 300 g for 3 minutes, low brake) before spiking and the start of the staining procedure.
Cord and adult blood should always be washed three times using 1x reagent D (3 x 2 mL at 300 g for 3 minutes, low brake) before spiking and the start of the staining procedure.
Proposed translations
(Polish)
| 1 +1 | powolne wyhamowanie |
geopiet
|
| 3 | niski poziom zwalniania/hamowania |
Frank Szmulowicz, Ph. D.
|
| 1 -1 | na niskich obrotach |
geopiet
|
Proposed translations
+1
1 hr
Selected
powolne wyhamowanie
.
4 KudoZ points awarded for this answer.
-1
27 mins
na niskich obrotach
odwirowanie próbki „na niskich obrotach ” ... ?
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Note added at 30 mins (2024-04-11 12:12:16 GMT)
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Centrifuge the collection tubes at 300 x g at room temperature (20°C to. 25°C) for 10 minutes with the low brake on. - https://sanguinebio.com/wp-content/uploads/2023/12/Whole-Blo... - page 2
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Note added at 38 mins (2024-04-11 12:20:37 GMT)
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The centrifuge brake setting is the other crucial parameter that greatly influences yield and purity. Rapid braking may cause rotational turbulence inside the tube during centrifuge slow down, causing cells to be washed off the pellet into the supernatant. This problem is more pronounced with wider centrifuge tubes, or when the tube is half full. Old centrifuges with a simple on-off brake setting should always have the brake off. Centrifuges with a high/low/off brake setting range may also have to be used in the off mode if the low brake speed is too fast. Centrifuges with a 1 to 10 scale of braking should be used with the brake set on 2 or less. The brake should always be off for the Ficoll spin.
- https://cdn.ach2.org.au/uploads/2020/08/IVRN_Lab_Manual_Upda... - page 7
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Note added at 30 mins (2024-04-11 12:12:16 GMT)
--------------------------------------------------
Centrifuge the collection tubes at 300 x g at room temperature (20°C to. 25°C) for 10 minutes with the low brake on. - https://sanguinebio.com/wp-content/uploads/2023/12/Whole-Blo... - page 2
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Note added at 38 mins (2024-04-11 12:20:37 GMT)
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The centrifuge brake setting is the other crucial parameter that greatly influences yield and purity. Rapid braking may cause rotational turbulence inside the tube during centrifuge slow down, causing cells to be washed off the pellet into the supernatant. This problem is more pronounced with wider centrifuge tubes, or when the tube is half full. Old centrifuges with a simple on-off brake setting should always have the brake off. Centrifuges with a high/low/off brake setting range may also have to be used in the off mode if the low brake speed is too fast. Centrifuges with a 1 to 10 scale of braking should be used with the brake set on 2 or less. The brake should always be off for the Ficoll spin.
- https://cdn.ach2.org.au/uploads/2020/08/IVRN_Lab_Manual_Upda... - page 7
Peer comment(s):
| disagree |
Andrzej Mierzejewski
: Przyspieszenie dośrodkowe: 300 x g = 3000 m/s2 oznacza bardzo wysokie obroty, nawet kilkanaście tysięcy na minutę,
21 hrs
|
|
niefortunne sformułowanie z mojej strony, mia łem na myśli „powolne”, czyli takie „przedłużone” zwalnianie
|
2 hrs
Frank Szmulowicz, Ph. D.
United States
United States
Native in English
&
Polish
Works in field
United States
On time, on target, on budget.
Native in: English 
, Polish
Works in: Polish to English, English to Polish, Russian to Polish, and 1 more.
Related KudoZ points
:
360
niski poziom zwalniania/hamowania
wybierz poziom hamowania (0-9)
poziom hamowania (brake) 0 odpowiada swobodnemu zwalnianiu
https://www.eppendorf.com/product-media/doc/pl/174046/Eppend...
Most of the larger benchtop centrifuges on the market as well as some small benchtop centrifuges offer what are called acceleration and deceleration (braking) ramps. Most small benchtop centrifuges also enable the user to switch between a normal brake and a soft/slow brake.
https://handling-solutions.eppendorf.com/fileadmin/user_uplo...
https://handling-solutions.eppendorf.com/sample-handling/cen...
poziom hamowania (brake) 0 odpowiada swobodnemu zwalnianiu
https://www.eppendorf.com/product-media/doc/pl/174046/Eppend...
Most of the larger benchtop centrifuges on the market as well as some small benchtop centrifuges offer what are called acceleration and deceleration (braking) ramps. Most small benchtop centrifuges also enable the user to switch between a normal brake and a soft/slow brake.
https://handling-solutions.eppendorf.com/fileadmin/user_uplo...
https://handling-solutions.eppendorf.com/sample-handling/cen...
Discussion
[cut]
When the brake is shut off, the centrifuge simply runs down until the rotor stops by itself. This way, the gradient will not be disturbed by sudden braking.
[cut]
.. we recommend lowering the deceleration ramp (usually it is not necessary to shut off the brake completely) to keep the pellet from swirling up.
- https://handling-solutions.eppendorf.com/sample-handling/cen...
- https://patents.google.com/patent/US4241866A/en
- https://www.hettichlab.com/fileadmin/user_upload/hettichlab.... - page 20
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Level 9 = shortest ramp-down time, ...Level 1 = long ramp-down time, Level 0 = unbraked ramp-down.
- https://www.hettichlab.com/downloadcenter/Products/Manuals/R... - page 19
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do wyboru 9 trybów rozpędzania i hamowania lub niehamowany czas zatrzymania,
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5564473/