Jun 6, 2016 08:12
7 yrs ago
English term
Subtracted Data vs nm
English to Russian
Science
Biology (-tech,-chem,micro-)
описание картинки: https://dropmefiles.com/TFkrx
In addition, multiple-antigen binding study was done using OctetRed to determine if FIT10-Ig was able to bind recombinant CTLA-4 and PD-1 simultaneously. Briefly, FIT10-Ig was immobilize on AR2G sensor at concentration of 10 μg/ml, followed by binding of CTLA-4Ig and then PD-1 (or PD-1 first, then CTLA-4Ig) in assay buffer (PBS pH 7.4, 0.1% BSA, 0.02% Tween), with concentration at 80 nM. At the end of the experiment, the surface was regenerated with 10 mM glycine at pH1.5 five times (Figure 7). This experiment shows that FIT10-Ig was able to bind PD-1 when it had already bound to CTLA-4, and vice versa, indicating that FIT10-Ig was able to bind both CTLA-4Ig and PD-1 simultaneously.
что здесь Subtracted Data vs nm по оси x и y
Спасибо!
In addition, multiple-antigen binding study was done using OctetRed to determine if FIT10-Ig was able to bind recombinant CTLA-4 and PD-1 simultaneously. Briefly, FIT10-Ig was immobilize on AR2G sensor at concentration of 10 μg/ml, followed by binding of CTLA-4Ig and then PD-1 (or PD-1 first, then CTLA-4Ig) in assay buffer (PBS pH 7.4, 0.1% BSA, 0.02% Tween), with concentration at 80 nM. At the end of the experiment, the surface was regenerated with 10 mM glycine at pH1.5 five times (Figure 7). This experiment shows that FIT10-Ig was able to bind PD-1 when it had already bound to CTLA-4, and vice versa, indicating that FIT10-Ig was able to bind both CTLA-4Ig and PD-1 simultaneously.
что здесь Subtracted Data vs nm по оси x и y
Спасибо!
Proposed translations
(Russian)
| 4 +1 | измеренные значения минус величина фона / нм |
Natalie
|
Proposed translations
+1
2 hrs
Selected
измеренные значения минус величина фона / нм
Насчет subtracted data написано в абзаце [00155]:
Aliquots of antigen samples were also simultaneously injected over a blank reference and reaction CM surface to record and subtract any nonspecific binding background to eliminate the majority of the refractive index change and injection noise.
Т.е. представленные данные соответствуют измеренным величинам минус фоновые значения
Что касается "нм", то это таки нанометры. В системе OctetRed величина связывания изменяется по увеличению оптической плотности (в нм) при связывании образца с сенсором. Нашла это в брошюрке с описанием системы OctetRed http://www.sibcb.ac.cn/cfmb/download/Octet_e.pdf на стр. 10, где написано:
Octet Binding Curve = rate of increase in optical thickness as the
sample binds to the sensor
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Note added at 3 hrs (2016-06-06 11:22:04 GMT)
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Я неудачно написала: оптическая плотность, конечно, не измеряется в нм - там более сложная формула. Но "нм" - нанометры
--------------------------------------------------
Note added at 3 hrs (2016-06-06 11:26:00 GMT)
--------------------------------------------------
Вот объяснение насчет нанометров:
The spectral pattern of
the reflected light therefore changes as a function of the optical
thickness of the molecular layer, i.e. the number of molecules
bound to the biosensor surface. This spectral shift is monitored at
the detector, and reported on a sensorgram as a change in wavelength
(nm shift). Monitoring the interference pattern in real time
provides kinetic data on molecular interactions.
http://csbi.mit.edu/instrumentation/ForteBio_App_Note_14.pdf
Aliquots of antigen samples were also simultaneously injected over a blank reference and reaction CM surface to record and subtract any nonspecific binding background to eliminate the majority of the refractive index change and injection noise.
Т.е. представленные данные соответствуют измеренным величинам минус фоновые значения
Что касается "нм", то это таки нанометры. В системе OctetRed величина связывания изменяется по увеличению оптической плотности (в нм) при связывании образца с сенсором. Нашла это в брошюрке с описанием системы OctetRed http://www.sibcb.ac.cn/cfmb/download/Octet_e.pdf на стр. 10, где написано:
Octet Binding Curve = rate of increase in optical thickness as the
sample binds to the sensor
--------------------------------------------------
Note added at 3 hrs (2016-06-06 11:22:04 GMT)
--------------------------------------------------
Я неудачно написала: оптическая плотность, конечно, не измеряется в нм - там более сложная формула. Но "нм" - нанометры
--------------------------------------------------
Note added at 3 hrs (2016-06-06 11:26:00 GMT)
--------------------------------------------------
Вот объяснение насчет нанометров:
The spectral pattern of
the reflected light therefore changes as a function of the optical
thickness of the molecular layer, i.e. the number of molecules
bound to the biosensor surface. This spectral shift is monitored at
the detector, and reported on a sensorgram as a change in wavelength
(nm shift). Monitoring the interference pattern in real time
provides kinetic data on molecular interactions.
http://csbi.mit.edu/instrumentation/ForteBio_App_Note_14.pdf
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Comment: "Спасибо!"
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