Glossary entry (derived from question below)
Serbian term or phrase:
mispriming
English translation:
\"mispriming\"/\"misprajming\"/pogrešno započinjanje
Added to glossary by
Radica
Jun 11, 2013 20:36
10 yrs ago
Serbian term
mispriming
Serbian to English
Medical
Biology (-tech,-chem,micro-)
Mikrobiologija
U pitanju je uputstvo za koriscenje kompleta za brzu prenatalnu dijagnostiku trizomije i aneuploidije polnih hromozoma
The enzyme is completely inactive at room temperature. This prevents MISPRIMING during Polymerase Chain Reaction set up. Activation is obtained during the 15 min. at 95ºC step before PCR cycling. This simplifies PCR set up and handling that can easily be done at room temperature.
The enzyme is completely inactive at room temperature. This prevents MISPRIMING during Polymerase Chain Reaction set up. Activation is obtained during the 15 min. at 95ºC step before PCR cycling. This simplifies PCR set up and handling that can easily be done at room temperature.
Proposed translations
(English)
| 4 | "mispriming"/"misprajming"/pogrešno započinjanje |
Nikola Bijelić, MD, PhD
|
| 5 -1 | lažan rezultat |
Milena Nikolić
|
Proposed translations
15 hrs
Nikola Bijelić, MD, PhD
Croatia
Croatia
Specializes in field
Croatia
Professional medical & pharma translator
Native in: Croatian 
Works in: English to Croatian, English to Serbian, Croatian to English, and 6 more.
Related KudoZ points
:
8
Selected
"mispriming"/"misprajming"/pogrešno započinjanje
Izvinjavam se za više odgovora, ali zapravo su svi odgovarajući. Mispriming dolazi od riječi "primer", (na srpskom često prevedeno "prajmer"). Koliko znam, ne postoji službeni prijevod ovog termina u znanstvenoj literaturi. Zato predlažem "mispriming" pod navodnicima ili po uzoru na "prajmer", "misprajming". Radi se o pogrešnom započinjanju kod lančane reakcije polimeraze.
Ako netko ima bolju informaciju o službenom prijevodu, neka javi, jer i mene zanima.
Mispriming is the result of a primer binding to an unintended template resulting in amplification. The amplicon can sometimes be the same size as the intended product, but is usually a different size when viewed following agarose gel electrophoresis. Mispriming occurs because of poorly optimised conditions or because we haven't checked whether our sequence will inadvertently bind to an entirely different target entity e.g. a region of the human genome instead of the intended virus genome.
Ako netko ima bolju informaciju o službenom prijevodu, neka javi, jer i mene zanima.
Mispriming is the result of a primer binding to an unintended template resulting in amplification. The amplicon can sometimes be the same size as the intended product, but is usually a different size when viewed following agarose gel electrophoresis. Mispriming occurs because of poorly optimised conditions or because we haven't checked whether our sequence will inadvertently bind to an entirely different target entity e.g. a region of the human genome instead of the intended virus genome.
Example sentence:
Sinteza obilježenog DNA markera provodi se metodom nasumičnog započinjanja (engl.random priming)...
4 KudoZ points awarded for this answer.
Comment: "hvala!"
-1
13 hrs
Milena Nikolić
Native in Serbian
fast and accurate
Native in: Montenegrin 
, Serbian (Variants: Montenegrin , Montenegrin )
Works in: English to Montenegrin, English to Serbian, Montenegrin to English, and 1 more.
lažan rezultat
prim
Precise or proper to the point of affectation; excessively decorous.
ovdje je u pitanju čitanje rezultata
Precise or proper to the point of affectation; excessively decorous.
ovdje je u pitanju čitanje rezultata
Peer comment(s):
| disagree |
Nikola Bijelić, MD, PhD
: Nažalost, to nije taj termin. Vidite: http://www.uq.edu.au/vdu/PDU_PCRPrimers.htm Pozdrav!
2 hrs
|
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