During the fermentation process virus tests concerning endogenous and adventitious virus must be performed in order to guarantee that the validated purification scheme eliminates potential contaminants. Purification factors for the inactivation and removal of potential viral contaminants of indi3 10 vidual elimination steps are in the range of 10 - 10 , depending on the types of virus to be eliminated. A definite recovery process should contain different steps which account to a 12 log reduction of retrovirus like the murine xenotropic virus.

https://www.esteve.org/wp-content/uploads/2018/01/138121.pdf


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2.2. Biological Synthesis
Biological synthesis of LA is usually a two-step process, where the first step is LA fermentation and the second step is product recovery and/or purification. Presently, approximately 90% of LA is produced through the fermentation process. LA fermentation requires the use of suitable microorganisms, substrates, and purification techniques. Several researchers have been working on the development of economically viable processes for the biological production of LA.

[LA = lactic acid]

https://www.mdpi.com/2311-5637/8/11/609

RECOVERY AND PURIFICATION PILOT PLANT
In this plant, we process the fermentation broths by carrying out the main DSP stages used in the industry including, but not limited to, separation, extraction and purification of the microbial metabolites of interest.

https://adlbiopharma.com/

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Advances in the discovery of novel pharmaceuticals, with improved therapeutic efficacy and reduced side effects, contributed for the fast development of pharmaceutical industries. Significant progresses in pharmaceuticals have been achieved by the optimization of both the upstream (production) and downstream (purification and recovery of the target product from complex media) processing, which include the search on new technologies and adaptation of the existing unit operations to increase efficiency, without the increase of the final product cost. In pharmaceutical industries, the lack of efficient downstream processes can seriously affect the success of a new product and even results in its failure.

https://www.ciceco.ua.pt/files/0000000000005_c1.pdf

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Entre las 5 mezclas de solventes orgánicos utilizadas el empleo de 70% 2 propanol, 55% 2 propanol y 70 % etanol dan los mejores resultados, obteniendo un rendimiento del 35% con un recobro de la proteína del 44%.

http://nutriciondebovinos.com.ar/MD_upload/nutriciondebovino...

5.4.4. RECUPERACIÓN.

También conocida como conversión o recobro del sistema ( recovery ), es el porcentaje de producto que se obtiene a partir de un determinado volumen de agua de alimentación:

https://upcommons.upc.edu/bitstream/handle/2099.1/6233/03_Me...

recobrar.

(Del lat. recuperāre).

1. tr. Volver a tomar o adquirir lo que antes se tenía o poseía. Recobrar las alhajas, la salud, el honor.

2. prnl. Recuperarse de un daño recibido.

3. prnl. desquitarse (‖ reintegrarse de lo perdido).

4. prnl. Volver en sí de la enajenación del ánimo o de los sentidos, o de un accidente o enfermedad.

https://www.rae.es/drae2001/recobrar



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Una de las tendencias actuales es utilizar los enfoques de integración e intensificación de bioprocesos para el desarrollo de sistemas de recuperación y purificación de productos biológicos, particularmente proteínas.

La industria farmacéutica demanda procesos biotecnológicos que le permitan la recuperación y purificación de proteínas mediante el uso de tecnologías escalables y eficientes.

https://www.redalyc.org/pdf/620/62040111.pdf